清洁指南

配药环境

微粒污染会堵塞MJ分配装置的孔口并干扰操作。因此,拥有一个干净的操作环境是必不可少的。
  • 避免在易受灰尘或静电吸引灰尘的区域处理配药液、配药管和配药装置。在相对湿度较低的条件下(不使用时,将分配装置、管道、液体容器和拭子存放在封闭的无尘区域),静电更为普遍。
  • 使用5微米或更小孔径的过滤器过滤来自气动控制系统的空气和将进入设备的所有液体。
  • 确保所有流体处理材料(即玻璃器皿、管道、过滤器等)已彻底清洁和/或用过滤水或适当溶剂冲洗。
  • 使用合适的实验服(实验服、手套)减少衣服或皮肤的污染。

分配器操作

MJ分配装置的孔口容易被喷射溶液中的颗粒物质堵塞。喷射液在使用前必须过滤。配药前,您应:
  • 使用前冲洗新的注射器过滤器,以去除制造过程中的颗粒。
  • Confirm the tubing and reservoir will withstand the solvents you choose. Otherwise dissolved material can contaminate the dispensing device.
  • 在分配之前,用过滤溶剂冲洗管道和储液罐,然后用过滤的压缩空气干燥。过滤异丙醇(或其他溶剂)具有比水更大的润湿能力,是去除微粒的更好选择。
  • Always clean the dispensing device before use. Back-flush the device by applying vacuum through the fluid connector and immersing the glass tip within a clean solvent. This back-flush method can also be used to remove orifice clogs. Warm solvents under low power ultrasonication work best, but understand the dangers of heating and sonicating volatile solvents. Do not to sonicate for more than 1 minute and never immerse more than the glass tip into the cleaning solution.

美赞臣配药设备必须在使用后立即清洗,以确保可靠的配药和延长使用寿命。注:在清洁之前,必须从MJ-AL型装置上拆除内联过滤器。
  • Use a wet clean foam swab or tissue wetted with water or solvent, to clean the exterior surface of the dispensing device, including the metal body and glass tip. Inspect under a low power microscope to confirm the glass tip surface is clean.中高音
  • 必须首先拧松并拆下MJ-AL型装置的过滤器。然后,在执行下面概述的清洁或堵塞孔口恢复程序之前,将装置重新连接到内螺纹鲁尔管接头(见右图)。在线过滤器部分应浸泡在适当的清洗液中。然后,应将过滤器连接到内螺纹鲁尔管接头上,并用清洗液从鲁尔管接头侧清洗,以去除污染物。在重新组装过程中,只能用手拧紧内螺纹鲁尔管接头、在线过滤器和装置主体,以避免过度拧紧和损坏。
  • 用1-3mL分配溶液的溶剂反冲洗设备,然后用2%水溶液(80℃)的Micro-90清洁溶液(Cole Parmer cat#U-18100-00)或同等溶液。使用低倍显微镜/立体镜检查分配装置的玻璃尖端。反冲洗1分钟后,观察分配装置的尖端,以验证是否已清除所有残留物、颗粒或冷凝液。重复此步骤,直到尖端清洁为止。最后,用去离子水或MilliQ水反冲洗尖端至少一分钟,然后用丙酮反冲洗一分钟。在储存或使用之前,先用真空吸尘器将设备内部干燥一分钟,然后将清洁后的设备在70℃的烘箱中干燥30分钟。
  • 在用2%水溶液(80ºC)的Micro-90清洗液清洗之前,可以用10%次氯酸钠溶液去除难处理的物质,如蛋白质、DNA、抗体等。如上所述冲洗并干燥设备。
  • 在反冲洗前,在清洗液中仅浸泡孔几分钟至一夜后,通常可以去除顽固颗粒。

孔口堵塞程序

一个孔堵塞,不能删除d using the cleaning and back-flushing procedures described above may be removed using the following protocols. Please note that leaving solutions in the device for extended periods of time, such as overnight, can result in orifice clogging.
  1. Immerse the tip of the device and backflush with a 2% solution (80°C) of Micro-90 Cleaning Solution (Cole-Parmer cat. # U-18100-00) in a beaker held in an Ultrasonic cleaner. Avoid the use of high power output Ultrasonic cleaners, as they can damage the fragile orifice. Also, minimize the amount of time the MJ dispensing device tip is exposed to Ultrasonic cleaning. Check the condition of the orifice after one minute. Repeat if necessary. A cotton swab can be used to wipe the tip of the device during this procedure to help free the clog.
  2. If the orifice remains clogged, immerse the tip of the device in a 5% water solution (80°C) of Micro-90 Cleaning Solution (Cole-Parmer cat. # U-18100-00) for overnight. Then, perform the backflush procedure described in “1” above.
  3. The MJ device can be returned to MicroFab if the cleaning procedures described above fail to unclog the orifice. The device will be cleaned, retested, and returned with a service fee applied. For each returned device fill out and attach the Dispensing Device Return Form (found under Support/Return Forms).注意:返回的每个设备都需要填写完整的表格。不要将多个设备合并到一个分配设备退货单上。

注意事项

不要将MJ分配装置的主体浸入Micro-90清洁溶液或其他溶剂中,因为这些液体会损坏PZT的环氧树脂和镍涂层。水和/或IPA可用于清洁MJ分配装置外壳。
避免使用高功率输出的超声波清洁器,因为它们会损坏孔的边缘。此外,尽量减少美赞臣点胶装置尖端暴露于超声波清洗的时间。


附加信息

清洁指南(PDF格式)